Journal: Gut Microbes

Article Title: A naturalized gut microbiome interacts with dietary fibers to protect against colonic inflammation

doi: 10.1080/19490976.2026.2649435

Figure Lengend Snippet: Measurements of NF-κB activity and systemic inflammation markers. (A) NF-κB activity in the liver of DSS-treated (left) and untreated (right) mice as average radiance of emitted bioluminescence as 1000 photons per second per area per steradian. (B) Relative expression of Il1b in the livers of DSS-treated animals relative to untreated animals as log2 of fold change. (C) μg lipopolysaccharide-binding protein (LBP) per mL plasma in DSS-treated (left) and untreated (right) mice measured after the first cycle of DSS treatment. (D) μg serum amyloid A (SAA) per mL plasma in DSS-treated (left) and untreated (right) mice measured after the second cycle of DSS treatment. Box plots show individual mice (dots/triangles), median (line), mean (+), IQR (box), and minimum to maximum (whiskers). Bar plots show individual mice (dots/triangles), mean (bar) and SD (whiskers). Dark blue/filled dots, Lab FR ( n = 2–3); light blue/open dots, Lab FL ( n = 5–10); brown/filled triangles, Fer FR ( n = 9); beige/open triangles, Fer FL ( n = 6–9). All untreated groups: n = 3–6. The p values for the effect of diet, housing condition, and interaction determined by two-way ANOVA are presented, while letters designate significant differences between groups following Tukey's multiple comparison testing (adjusted p -value ≤ 0.05). Statistics performed on ◊, log 2 ; or ∫, log 10 transformed data. DSS, dextran sulfate sodium; Lab, laboratory; Fer, feralized; FR, fiber rich; FL, fiber low; FC, fold change; NF-κB, nuclear factor kappa-light-chain-enhancer of activated B cells.

Article Snippet: Plasma SAA was measured with Mouse Serum Amyloid A DuoSet ELISA (R&D Systems, #DY2948-05), and lipopolysaccharide binding protein (LBP) was measured with a Mouse LBP ELISA Kit (Biorbyt, #orb391103) following the manufacturers' protocols.

Techniques: Activity Assay, Expressing, Binding Assay, Clinical Proteomics, Comparison, Transformation Assay

Journal: Gut Microbes

Article Title: A naturalized gut microbiome interacts with dietary fibers to protect against colonic inflammation

doi: 10.1080/19490976.2026.2649435

Figure Lengend Snippet: Levels of plasma and fecal inflammatory biomarkers following DSS-induced colitis in FMT recipients. (A) Fecal levels of Lipocalin-2 (LCN2), (B) plasma levels of Serum amyloid A (SAA) and (C) lipopolysaccharide-binding protein (LBP) were determined by ELISA. Box plots show median (line), mean (+), IQR (box) and minimum to maximum (whiskers). Green/filled diamonds, R-Sham ( n = 6–11); blue/open diamonds, R-Lab ( n = 5–12); orange/open square, R-Fer ( n = 7–12). Letters designate significant (adjusted p -value ≤ 0.05) differences between groups determined by one-way ANOVA following Tukey multiple comparison test. Statistics performed using ◊, log2; or §, square root transformed data. R, recipient; Lab, laboratory; Fer, feralized; FMT, fecal microbiota transfer.

Article Snippet: Plasma SAA was measured with Mouse Serum Amyloid A DuoSet ELISA (R&D Systems, #DY2948-05), and lipopolysaccharide binding protein (LBP) was measured with a Mouse LBP ELISA Kit (Biorbyt, #orb391103) following the manufacturers' protocols.

Techniques: Clinical Proteomics, Binding Assay, Enzyme-linked Immunosorbent Assay, Comparison, Transformation Assay

Journal: Journal of Neuroinflammation

Article Title: Single-cell RNA sequencing uncovers neutrophil clusters associated with autoimmune neuroinflammation

doi: 10.1186/s12974-026-03772-9

Figure Lengend Snippet: Enhanced SAA1 expression and NET formation in plasma of MS patients. (A) SAA1 protein expression was quantified by ELISA in plasma samples collected from MS patients and HC. (B) H3.1-nucleosome expression was quantified by ELISA in plasma samples collected from MS patients and HC

Article Snippet: The human SAA1 ELISA kit (DY3019-05; R & D Systems, Minneapolis, MN) detected human SAA1 in plasma samples from MS patients and HC.

Techniques: Expressing, Clinical Proteomics, Enzyme-linked Immunosorbent Assay